transformation of canola (r line hyola 308) by pgip1 gene from bean cv. daneshjoo to improve resistance to sclerotina sclerotiorum
نویسندگان
چکیده
during plant infection, fungal pathogens secret polygalacturonase (pg) that are capable of degrading cell wall of susceptible plant tissues. polygalacturonase-inhibiting proteins (pgips) are able to specifically inhibit fungal polygalacturonases (pgs) activity. the inhibition of fungal pgs by pgips suggests that pgips have a role in plant tolerance to fungal infections. in this study bean (daneshjoo cultivar) pgip1 gene was overexpressed under the control of the camv 35s constitutive promoter in brassica napus, r line hyola 308. transformation of cotyledonary petioles was achieved via agrobacterium tumefaciens lba4404. after infection with agrobacterium, the explants were transferred to selected regeneration medium. the transformed explants were screened in kanamycin containing media. the explants were excised and rooted using appropriate growth regulator. putative transgenic lines obtained from independent transformation events transferred to the greenhouse for hardening. the presence of pgip1 in putative transgenic lines confirmed by the polymerase chain reaction (pcr) using specific primers. also, the results of bioassay demonstrated that the lesion sizes occurred on leaves of transgenic canola by sclerotina sclerotiorum (causal agent of canola stem rot) were significantly retarded in compared to non transgenic canola plant using leaf detached assay.
منابع مشابه
انتقال ژن pgip1 لوبیا رقم دانشجو به کلزا (رقم R line Hyola 308) جهت ایجاد مقاومت علیه قارچ Sclerotina sclerotiorum عامل پوسیدگی ساقه
یکی از روشهای ممانعت از نفوذ قارچهای بیماریزا به درون گیاهان، مهار فعالیت آنزیمهای پکتینازی (مانند پلیگالاکتوروناز) این قارچها میباشد. پروتئینهای مهارکننده فعالیت آنزیم پلیگالاکتوروناز (PGIP) از جمله پروتئینهایی هستند که توسط برخی از گیاهان بیان میشوند و قادرند قدرت نفوذ قارچ به گیاه را مهار نمایند. در این تحقیق جهت ایجاد مقاومت به قارچ Sclerotina sclerotiorum در گیاه کلزا، ژن pgip1 ب...
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متن کاملانتقال ژن pgip1 لوبیا رقم دانشجو به کلزا (رقم r line hyola 308) جهت ایجاد مقاومت علیه قارچ sclerotina sclerotiorum عامل پوسیدگی ساقه
یکی از روش های ممانعت از نفوذ قارچ های بیماریزا به درون گیاهان، مهار فعالیت آنزیم های پکتینازی (مانند پلی گالاکتوروناز) این قارچ ها می باشد. پروتئین های مهارکننده فعالیت آنزیم پلی گالاکتوروناز (pgip) از جمله پروتئین هایی هستند که توسط برخی از گیاهان بیان می شوند و قادرند قدرت نفوذ قارچ به گیاه را مهار نمایند. در این تحقیق جهت ایجاد مقاومت به قارچ sclerotina sclerotiorum در گیاه کلزا، ژن pgip1 ب...
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عنوان ژورنال:
علوم گیاهان زراعی ایرانجلد ۴۲، شماره ۱، صفحات ۵۳-۶۱
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